Determination of Cariogenic Compounds in Saliva after Eating Apple
김지은, 권하정, 김남중, 황경숙,
소속 상세정보
김지은 ( Kim Ji-Eun ) - Kyung Hee University School of Dentistry Department of Preventive and Social Dentistry & Institute of Oral Biology
권하정 ( Kwon Ha-Jeong ) - Kyung Hee University School of Dentistry Department of Preventive and Social Dentistry & Institute of Oral Biology
김남중 ( Kim Nam-Jung ) - Shinhan University Department of Dental Technology
황경숙 ( Hwang Kyung-Sook ) - Shinhan University Department of Dental Technology
KMID : 1157520140100040209
Abstract
Objective: The purpose of this study is to analyze the concentration of organic acids and sugars in saliva after eating apple to quality assessment of causing dental caries.
Methods: Unstimulated saliva (0.5 ml) was collected before and after 0, 1, 2, 5, 10, 20, and 30 minutes of apple intake. The prepared saliva samples were analyzed by chromatographic method using high-performance liquid chromatography (for organic acids analysis) and Ion chromatography-pulsed amperometric detection (for sugars analysis).
Results: Organic acids are remained up to 30 minutes after eating apple. The concentrations of malic acid and succinic acid were increased until 2 minutes after apple intake, decreased from 2 minutes to 10 minutes, and rebounded after 10 minutes. The citric acid concentration reached its peak at 1 minute after apple intake. Sugar concentrations (glucose, fructose and sucrose) shown the highest level immediately after apple intake, and sharply decreased as time elapsed.
Conclusion: Apple could be a cause of dental caries by remaining the organic acids for long time up to 30 minutes. It is necessary to cleaning mouth after apples in order to decreasing the salivary concentrations of cariogenic compounds.
키워드
apple; organic acids; sugars; chromatography
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